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. Author manuscript; available in PMC: 2012 Jan 7.

Published in final edited form as: J Mol Biol. 2010 Oct 26;405(1):185–200. doi: 10.1016/j.jmb.2010.10.029

Table 1.

Amino acid residue identities of selected I-SceI variants

Target Site	Isolate	Library
		3 var				5 var
		Amino Acid Position
A/T₊₄ (WT)		61	88	100	^a#	59	61	86	88	100	^a#
	WT	Glu	Arg	Gly	6	Gln	Glu	Lys	Arg	Gly	10
	G100A	Glu	Arg	Ala	3	Gln	Glu	Lys	Arg	Ala	5
C/G₊₄	WT	Glu	Arg	Gly	6	Gln	Glu	Lys	Arg	Gly	6
	G100T	Glu	Arg	Thr	5
	G100A					Gln	Glu	Lys	Arg	Ala	6
	K86R/G100T					Gln	Glu	Arg	Arg	Thr	3
	K86R/G100C					Gln	Glu	Arg	Arg	Cys	4
	K86R/G100S					Gln	Glu	Arg	Arg	Ser	5

^a

The number of isolates containing each combination of residues is indicated.