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ArgBP2-Flag (full-length) binding to major cardiac proteins.
A. Cardiac muscle proteins binding to ArgBP2-Flag-M2-anti-Flag complex. Recombinant ArgBP2-Flag protein was allowed to bind to M2 mouse anti-Flag resin. HTE extract was incubated with M2 resin alone (left lane) or with the complex ArgBP2-Flag-M2-resin (right lane).
B. ArgBP2 protein purified from 8 M urea extract effectively binds to cardiac muscle proteins after reconstitution into the binding buffer.
