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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov 10;67(12):808–823. doi: 10.1002/cm.20490

Figure 11.

Figure 11

(A, B) Western blots demonstrating full length ArgBP-2-Flag binding to (A) α-actinin in lane 1 and (B) vinculin in lane 2. (A, B) Note that the anti-FLAG antibodies alone do not pull down α-actinin (A, lane 2) or vinculin (B, lane 1) in the absence of ArgBP2. Lanes three in (A, B) represent the gel positions of purified (A) α-actinin and (B) vinculin. (C) Western blots with α-actinin antibody staining of the coprecipitation of purified fusion protein (lane 1) GST-midArgBP (ArG15) and (lane 2) ArgBP-SH3 (Arg-21) with α-actinin. Lane 1 demonstrates binding of ArgBP-SH3 (lane 1) and ArgBP-SH3 (lane 2) and α-actinin. Lane three indicates that free GST does not pull down α-actinin. Lane 4: purified α-actinin sample unreacted with fusion protein. (D) Similar Western blot using the same fusion proteins in (C) but testing for the binding of purified vinculin. Lane 1 demonstrates binding of ArgBP-SH3 (lane 1) and ArgBP-SH3 (lane 2) with vinculin. Lane three indicates that free GST does not pull down vinculin. Lane 4: purified vinculin sample unreacted with fusion protein.