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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov 10;67(12):808–823. doi: 10.1002/cm.20490

Figure 3.

Figure 3

(A) ArgBP2 containing Triton X-100 extract from Sf9 cell was used directly to purify ArgBP2-Flag protein using Ni resins as described in the Materials and Methods. Lane 1 was the void volume. Lanes 2 and 3 show the eluted single band of ArgBP2. This single 80 kDa band was excised from the gel and used for immunization to produce polyclonal antiserum.

(B). Differently extracted samples of adult hearts were subjected to SDS-PAGE followed by the Western blot analysis. Lane 1- HTE- soluble fraction (Heart Triton X-100 Extract); Lane 2 – RIPA-soluble fraction; Lane 3, recombinant ArgBP2 protein was used as a standard. Note the presence of two isoforms of ArgBP2 in adult hearts (80 and 120 kDa).