Representative images of cardiomyocytes cultured from 10-day quail embryo and transfected with ArgBP2-YFP. (A, B, C, F, and green in E, H) ArgBP2 targets to (A) Z-bodies of pre- and nascent myofibrils and (B) Z-bands of mature myofibrils. (C-H) Overexpression of ArgBP2-YFP leads to the accumulation of spherical aggregates of ArgBP2-YFP protein that do not react with antibodies to Z-band proteins as shown for alpha-actinin (D and red in E), and vinculin (G, red in H). Bars = 5 microns (A, B) and 10 microns (C-H).