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ArgBP2A and titin in cardiomyocytes. Cells were transfected with GFP-ArgBP2A (A, D, G) and then fixed, permeabilized, and stained with an anti-titin (zeugmatin) antibody (B, E, H). The titin antibody colocalizes with the GFP-ArgBP2A in the Z-bands (C, F, I), but the GFP-ArgBP2A containing Z-bodies of the forming myofibrils do not stain for titin (B, C). (D-I). Overexpression of the GFP-ArgBP2A leads to the loss of myofibrils. Bars = 10 microns.
