Figure 2.

K-means clustering of differential gene expression. K-means clustering (clusters = 10, average linkage, Euclidian distance) was employed to identify functional relationships between genes. Several clusters were identified with greater similarity between females with different withdrawal phenotype (none were identified showing greater similarity between both genders of a single selected line), suggesting a divergent transcriptional response to EtOH in females vs males. (a) Clustering identified genes with a sexually dimorphic response to withdrawal from chronic EtOH exposure, with upregulation in females, and downregulation in males. (b) Female upregulated genes (upregulation in female WSP and WSR). (c) Female downregulated genes (downregulation in female WSP and WSR). Additional clusters can be seen in Supplementary Figure 3 in supporting information online. No clusters were specifically identified as phenotype-divergent (eg none demonstrated clustering of male and female WSP).