Figure 7.

Purified recombinant Rrd1 dissociates Rpb1 from the chromatin in vitro. Increasing amounts of purified HIS-Rrd1 were added to the chromatin fraction isolated from rrd1Δ mutant strain expressing Apn1-MYC and incubated at 30°C for 1 h in phosphate buffer. Chromatin was recovered from the buffer and both fractions were analyzed by Western blotting probed with 4H8 (against Rpb1) and anti-MYC antibodies. Apn1-MYC was used as loading control. Result shown is representative of at least three experiments.