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. 2011 Jan 11;6(1):e15867. doi: 10.1371/journal.pone.0015867

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Inclan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Intracellular cAMP levels were measured in the presence (2 mM) or absence of calcium in (A) PAO1, (B) PAO1ΔcpdA, or (C) PA103. Gene names denote in-frame deletions. PAO1ΔcpdA(comp) fimL-FL denotes complementation of PAO1ΔcpdAΔfimL with fimL-3X-FLAG by gene replacement in the fimL locus. PAO1ΔcpdAΔcyaB (CTX-cyaB) denotes complementation of PAO1ΔcpdAΔcyaB with cyaB at the CTX phage attachment site. Note the scale differences. Shown are mean results of triplicate samples from 3 experiments. Error bars indicate SD (for cpdA in (A) +/−4.7 in the presence of Ca²⁺ and +/−3.6 in the absence of Ca²⁺). (***) P<0.001 compared to the wild type strain grown in the absence of calcium.