Figure 2. Loss of fimL does not affect cyaB transcription or protein levels.

(A) β-galactosidase activity was measured in the presence or absence of calcium in PAO1 or PAO1ΔfimL. All strains carry a lacZ transcriptional reporter integrated at the CTX site without a promoter (P₀-lacZ) or with the cyaB promoter (PcyaB-lacZ). Error bars indicate SD of the average rate from 14 data points taken from two experiments. (B) Immunoblot of PAO1 or PAO1ΔfimL in which the wild type cyaB gene has been replaced with cyaB-His. Lysates were prepared from bacteria grown in the presence or absence of Ca²⁺ and probed with anti-His antibody (upper panel) or anti-PilA antibody (lower panel) as a loading control.