Global DNA demethylation results in TG2 re-expression in cultured glioma cell lines. a The glioma lines U87MG, U118, T98G and A172 were cultured in the presence or absence of the global DNA demethylating agent 5-azadC as indicated. Total RNA was isolated, cDNA synthesized, and PCR conducted using TG2 specific primers (top) or GAPDH specific primers (bottom). b. Protein extracts were obtained from the glioblastoma cell lines U118, T98G and A172 cultured in the presence or absence of 5-azadC. Cell extracts were immunoblotted with anti-TG2 (top) or anti-tubulin (bottom)