Figure 3.

The G5 + G6 or G10 GATA-1 binding sites alone are not required for GATA-1-stimulated transcription of the p21 promoter. Luciferase reporter assays were performed in HeLa cells as in Figure 1 with −4.6 kb p21 promoter-reporter plasmids containing either the wild-type (wt) sequence or one in which the G5 + G6 sites (A) or the G10 site (B) was mutated (WGATAR → WTATAR). Error bars indicate the standard deviations of triplicate assays. Similar results were obtained in three experiments.