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. Author manuscript; available in PMC: 2012 Jan 28.
Published in final edited form as: J Mol Biol. 2010 Nov 12;405(4):926–938. doi: 10.1016/j.jmb.2010.10.056

Figure 4.

Figure 4

Isothermal titration calorimetry and in vivo assays with the SMK box. (a) Thermogram generated upon titration of SAM into SMK51. Fitting to a one-site model reveals a ΔH of -18.7 ± 0.2 kcal/mol and a KD of 63 ± 11 nM. (b) Analogous data for SMK59, with a ΔH of -10.7 ± 0.3 kcal/mol and a KD of 400 ± 78 nM. (c) Expression of Pgly-metK-lacZ fusions in B. subtilis during growth under high SAM (+Met) or low SAM (Met) conditions. β-galactosidase activities are expressed in Miller units. Induction ratio is the β-galactosidase activity in cells grown in the absence of methionine divided by the activity in cells grown in the presence of methionine.