. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Med Res Rev. 2010 Feb 4;31(4):483–519. doi: 10.1002/med.20187

Table I.

Uptake and Inhibition Kinetic Parametersof Resealed Bovine Chromaffin Granule Ghosts/Membranes

Substrate/Inhibitor		K_m or K_I (μM)	V_max (nmoles/min.mg)	K_D (nM)	Ref.
1	5HT	19 ± 4	1.6 ±1.0		32 ^a
2	DA	25 ± 7	0.8 ± .5		32 ^a
		23.8 ±1.5	1.60±0.03		33 ^b
3	NE	91.5 ±10.5	0.95±0.05		33 ^c
4	E	68.4 ±4.7	1.13±0.03		33 ^c
5	TBZ	3.2 ×10⁻³		1.3	34 ^c
6	RES	10 × 10⁻³		0.3 ± 0.2 (I)	35 ^d
				18 ±10 (II)
7	KET			45 ± 2.4	36 ^e
8	TBZOH	3.7 × 10⁻³		2.9	34 ^c
				5.8 ± 1.0	35 ^d
16				5.5 × 10⁻³	93 ^f

Determined using resealed granule ghosts using [³H]5HT and [³H]DA in the presence of 100 mM ATP, at pH 7.2³².

Determined with resealed granule ghosts using non-radioactive substrates with HPLC-EC in the presence of 10 mM ATP, at pH 7.2³³.

Determined with [³H]TBZ and [³H]TBZOH at 25 °C³⁴ using bovine granule membranes.

Determined with [³H]TBZOH and [³H]reserpine (sites I & II) using bovine chromaffin granule membrens³⁵.

Determined with [³H]KET at 30 °C using granule memebranes³⁶.

K_d determined using [³H] ketanserin at 0° C and chromaffin granule memebranes⁹³.