. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Med Res Rev. 2010 Feb 4;31(4):483–519. doi: 10.1002/med.20187

Table V.

Inhibition Kinetic Parameters of MPP⁺-APP Conjugates for Bovine Chromaffin Granule VMAT.^a


Inhibitor	K_i (μM)^a	K_m,_DA/K_i,I^b	Inhibitor	K_i (μM)^a	K_m,_DA/K_i,I^b
59	38.4 ± 4.2	0.9	64	2.8 ± 0.1	8.7
60	53.0 ± 13.6	0.7	65	0.4 ± 0.1	55
61	16.4 ± 2.7	1.8	66	0.5 ± 0.1	41.5
62	13.4 ± 1.7	2.7	67	4.5 ± 0.5	11.3
63	1.4 ± 0.2	24.1

Determined with resealed granule ghosts using non-radioactive substrates with HPLC-EC quantification of catecholamine uptake in the presence of 10 mM ATP, at pH 7.2.

Included for comparison purposes (for further details see Table IV foot notes and ref. 55).