A myenteric ganglion of a Brazilian non-chagasic, non-megacolonic male patient aged 75 years stained for human neuronal protein Hu C/D (HU), neuronal nitric oxide synthase (NOS) and common choline acetyltransferase (ChAT). Exemplarily for all figures, the following colour combinations can be seen in the merged picture columns (left, respectively): NOS/HU-neurons appear orange (filled arrows in a, a′; empty arrow in a″), ChAT/HU-neurons turquoise (filled arrowheads in a, a″; empty arrowhead in a′), neurons co-immunoreactive for all three markers pink (triangles in a, a′, a″). A neuron reactive for HU but non-reactive for both NOS and ChAT is marked in Fig. 5a. Bar 50 μm