Table 2.
Reactivity of mAb 4B3-A11 and mAb 6A2-B1 with CHO K1 cells expressing various CIDR1 domains
| CHO cells | Fluorescence intensity (median)
|
||
|---|---|---|---|
| mAb 4B3-A11 | mAb 6A2-B1 | Anti-Tag (mAb 179) | |
| Control CHO | 3.3 | 3.0 | 2.5 |
| K1 | |||
| MC 179 | 85.7 | 541 | 599 |
| MC CIDR1 | 41 | 932 | 1,056 |
| ItA4 CIDR1 | 7.7 | 14.5 | 498 |
| A4tres CIDR1 | 9.0 | 26.7 | 569 |
| FVO CIDR1 | 4.3 | 9.8 | 77 |
| FCR3-CSA | 3.9 | 4.7 | 1,259 |
| CIDR1 | |||
Quantitative binding of mAb 4B3-A11 and mAb 6A2-B1 to CHO K1 cells expressing various CIDR1 domains represented by the median fluorescence intensity from Fig. 2. Expression levels of each CHO K1 clone were measured by using mAb 179.