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. Author manuscript; available in PMC: 2011 Jan 12.
Published in final edited form as: IEEE NIH Life Sci Syst Appl Workshop. 2009 May 2;2009:9–12. doi: 10.1109/LISSA.2009.4906696

Fig. 5.

Fig. 5

Illustration of constructing pRNA multimers. A. pRNA chimeric monomer with 3′-end annealed oligo which conjugate the detection molecule and chemical ligand at the same time. B. a. Twin dimer formed through palindrome sequence; b. Dimer formation through loop-loop interaction. C. Tetrameric RNA chimera. D. pRNA Trimer. E. Polyacrylamide gel showing pRNA multimers in native (upper panel) and denatured (lower panel) condition. RNA nomenclature: To simplify the description of RNA construction and multimer assembly, uppercase letters will be used to represent the right hand loop of pRNA and lowercase letters to represent the left hand loop. The same letters in upper and lower cases indicate complementary sequences for loop/loop interaction, while different letters indicate non-complementary loops. For example, pRNA A-b′ represents pRNA where right loop A (5′G45G46A47C48) is complementary to left loop a′ (3′C85C84U83G82) of pRNA B-a′. And left loop b′ (3′U85G84C83G82) of A-b′ pRNA is complementary to the right loop B (5′A45C46G47C48) of pRNA B-a′.