Figure 2.

Silent mutant LAMR protein expression and cell phenotype. (a) Western blot analysis of LAMR protein expression in WT, silMUT, and silMUT²²⁰ cells 4 days after transfection with siLAMR and siGLO. Antibodies against LAMR (top panel) and FLAG (bottom panel) were used to detect expression of endogenous and exogenous LAMR protein expression levels, respectively. Full-length silMUT LAMR runs slightly higher than endogenous LAMR (37 kDa) and can be detected with antibodies for LAMR (arrow). silMUT²²⁰ is not detected by the antibody used for LAMR and runs at ∼25 kDa. Blots represent three separate transfections. (b) Images of WT, silMUT, and silMUT²²⁰ cells treated with siLAMR and siGLO 4 days after transfection. Images are indicative of at least three separate transfections