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Functional intracellular persistence of HIV-1 inhibitors. Cells exposed to 100× the EC95 levels of each compound for 24 h were washed to deplete extracellular drug, cultured for various time periods (0, 2, 4, 8, or 12 h), exposed to HIV-Luc, and then further cultured for 48 h prior to cell lysis. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared to the no-drug control (based on Student's t test).
