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. 2010 Oct 18;55(1):395–398. doi: 10.1128/AAC.00970-10

FIG. 2.

FIG. 2.

Confocal microscopy analysis of the bacterial morphology and membrane permeability of vancomycin-susceptible E. faecalis JH2-2, VanA-type E. faecalis BM4316, or VanB-type E. faecalis BM4275. Cells were incubated for 1 h in the presence of 1× MIC of vancomycin (Vm) or daptomycin (D) or without antibiotic and stained by using a BacLight Live/Dead kit. Green-fluorescent bacteria have an intact membrane; red-fluorescent bacteria have a permeabilized membrane. Dysmorphic bacterial chains (arrows) were only observed after incubation in the presence of daptomycin.