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. 2010 Oct 18;55(1):395–398. doi: 10.1128/AAC.00970-10

FIG. 3.

FIG. 3.

Relationship between the decrease in bacterial counts (expressed as Δlog10 CFU/ml) and the rates of permeabilization of bacteria after exposure of vancomycin-susceptible E. faecalis JH2-2 (black symbols), VanA-type E. faecalis BM4316 (white symbols), or VanB-type E. faecalis BM4275 (gray symbols) to vancomycin (squares), daptomycin (circles), telavancin (triangles), or PA1409 (diamonds) at 4× MIC for 180 min. Permeabilization was studied by flow cytometry after staining using a BacLight Live/Dead kit. Bacterial counts were determined by enumeration of surviving bacteria on BHI agar. Each experiment was performed 4 times, and data are expressed as the mean (± standard deviation) percentages (%) of permeabilized bacteria and the mean (± standard deviation) difference between log10 CFU/ml counts obtained with and without antibiotic exposure.