FIG. 1.
M. tuberculosis Ddl inhibition and binding assays. Inhibition assays were carried out with purified Ddl as described in Materials and Methods. Enzyme activity was assayed by either the radiometric method (A) or the continuous Pi assay (B to E). IC50s were defined as the inhibitor concentration that decreased the enzyme activity by 50% and were calculated using regression and graphic analyses, collecting data points (each point represents the mean ± the standard error of the mean [SEM]; n ≥ 3) obtained at specified inhibitor concentrations (A), spanning the range from 1 nM DCS to 5 mM DCS (B), or observed in the linear range of the DCS dose-response curves (C to E).