TABLE 2.

Details of the DNA primers used in this investigation

Gene amplified or primer set (purpose or description)	Forward primer	Reverse primer	Amplimer size (bp)
16S rRNA	5′-GAGAAGAACGAGTGTGAGAG-3′	5′-CTAACACCTAGCACTCATCG-3′	391
patA (sequencing)	5′-TCTTGCTCAGTCCATCATCGAATAT-3′	5′-CCGCTGTGGATTAGTTCATTTCC-3′	2,963
patB (sequencing)	5′-AGAATCCAGTCCAGCGAAAGCT-3′	5′-GAAAGAACGACCAGATGTTCCAAT-3′	2,959
patA (expression)	5′-ATGTTGTCCTCGCAGCCTAT-3′	5′-ACGAACCGATGAACAAGAGG-3′	212
patB (expression)	5′-TTGCTGGTTCGGCTGTACTT-3′	5′-AACTGCTGTCATCTGGCCTT-3′	330
hexA (expression)	5′-GAGAATGCTCGCTCAGGTAA-3′	5′-TCACATGAGGAGCTTCAGGA-3′	421
1 (patA forward primer + transposon primer)	5′-TCTTGCTCAGTCCATCATCGAATAT-3′	5′-CCGGGGACTTATCAGCCAACC-3′	1,445
2 (patA reverse primer + transposon primer)	5′-CCGCTGTGGATTAGTTCATTTCC-3′	5′-CCGGGGACTTATCAGCCAACC-3′	1,566
3 (patB forward primer + transposon primer)	5′-AGAATCCAGTCCAGCGAAAGCT-3′	5′-CCGGGGACTTATCAGCCAACC-3′	1,487
4 (patB reverse primer + transposon primer)	5′-GAAAGAACGACCAGATGTTCCAAT-3′	5′-CCGGGGACTTATCAGCCAACC-3′	1,520