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. 2010 Oct 18;55(1):165–173. doi: 10.1128/AAC.01144-10

FIG. 4.

FIG. 4.

Oxidative burst of neutrophils is significantly enhanced in response to UK-66P-opsonized S. aureus. The oxidative burst activity of native mouse blood neutrophils was determined using a DHR123/R123 assay and flow cytometric analysis. (A) Oxidative burst was monitored by observing the fluorescence events (M1) in an FL1 overlay histogram. Wild-type S. aureus MA12-stimulated neutrophils with the addition of saline, isotype control antibody (IC), or UK-66P at concentrations of 0.3 mg/ml and 0.6 mg/ml. (B) As a specificity control for UK-66P, the oxidative burst was additionally monitored for IsaA mutant S. aureus MA12 ΔisaA-stimulated neutrophils with the addition of saline, IC, or UK-66P at concentrations of 0.3 mg/ml and 0.6 mg/ml. (C) Mean fluorescence intensity (MFI) of UK-66P-opsonized bacteria at concentrations of 0.3 and 0.6 mg/ml (equivalent to 15 mg/kg and 30 mg/kg body weight, respectively) compared to those for IC- or saline-opsonized wild-type and IsaA mutant bacteria. Significant differences are denoted (Mann-Whitney test). Error bars represent SDs.