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. 2010 Oct 18;55(1):165–173. doi: 10.1128/AAC.01144-10

FIG. 5.

FIG. 5.

Effect of UK-66P on survival of S. aureus within neutrophils in whole mouse blood. Mouse blood (100 μl) was incubated with 5 × 107 CFU of UK-66P-opsonized wild-type S. aureus MA12 or IsaA mutant S. aureus MA12 ΔisaA. Controls included isotype control antibody (IC)-opsonized bacteria. The number of neutrophil-associated CFU was determined by serial dilution and plating on TSB. UK-66P-opsonized wild-type S. aureus bacteria were killed significantly better than IC-opsonized bacteria (mean CFU ± SD, 1.13 × 105 ± 9.38 × 103 and 2.99 × 105 ± 3.65 × 103, respectively; Mann-Whitney test, P = 0.0286). UK-66P- and IC-opsonized IsaA mutant S. aureus produced similar results (mean CFU ± SD, 1.8 × 105 ± 1.3 × 104 and 1.9 × 105 ± 6.1 × 103, respectively). Error bars represent SDs.