TABLE 1.
Summary of isolation data, resistance characteristics, and identification of isolates used in this study
| Isolate | Origina | Resistance patternb | Phenotypic characterization | Molecular characterizationc |
|---|---|---|---|---|
| 60 | DB | PEN, ERY, RIF | S. sanguinis | S. parasanguinis (98.6) |
| 79 | AF | ERY, CLI, SXT, RIF | S. sanguinis | S. oralis (97.6) |
| 113 | WS | PEN, RIF | S. oralis | S. oralis (98.1) |
| 395 | AF | RIF | S. sanguinis | S. gordonii (98.9) |
| 745 | AF | ERY, CLI, TET, SXT, RIF | S. sanguinis | S. oralis (98.4) |
| 779 | BL | RIF | S. mitis | S. mitis (98.7) |
| 889 | B | PEN, SXT, RIF | S. parasanguinis | S. parasanguinis (97.4) |
| 971 | E | ERY, TET, RIF | S. sanguinis | S. parasanguinis (95) |
DB, duodenal biopsy specimen; AF, ascitic fluid; WS, wound swab; BL, bronchoalveolar lavage fluid; B, blood; E, eye.
PEN, intermediate or highly resistant to penicillin (MIC ≥ 0.25 μg/ml); TET, resistant to tetracycline (MIC ≥ 8 μg/ml); ERY, resistant to erythromycin (MIC ≥ 1 μg/ml); CLI, resistant to clindamycin (MIC ≥ 1 μg/ml); SXT, resistant to trimethoprim-sulfamethoxazole (MICs ≥ 4 and 76 μg/ml); RIF, resistant to rifampin (MIC ≥ 4 μg/ml).
The species identification was based on clustering with type strains in a phylogenetic tree obtained with concatenated partial sequences of 16S rRNA genes, sodA, rpoB, and hlpA. Numbers in parentheses indicate the percentage of identity with the corresponding type strain.