TABLE 2.

Kinetic parameters for nucleotide incorporation opposite template base dG at 37°C

DNA polymerase	dNTP	kp (s−1)	Kd (μM)	kp/Kd (μM−1 s−1)	Selection factora
Pol β	dCTP	5.02 ± 0.07	0.71 ± 0.04	7.1
	L-3TC-TP	0.00390 ± 0.00010	0.18 ± 0.02	2.2 × 10−2	325
	L-FTC-TP	0.027 ± 0.001	11 ± 2	2.5 × 10−3	2,900
Pol λ	dCTPb	1.57 ± 0.04	0.9 ± 0.1	1.7
	L-3TC-TP	0.00402 ± 0.00008	0.106 ± 0.010	3.8 × 10−2	46
	L-FTC-TP	0.0049 ± 0.0001	0.36 ± 0.03	1.4 × 10−2	120
Pol η	dCTP	49 ± 2	25 ± 4	2.0
	L-3TC-TP	0.0296 ± 0.0009	3.3 ± 0.3	9.0 × 10−3	220
	L-FTC-TP	0.0244 ± 0.0004	2.7 ± 0.1	9.0 × 10−3	220
Pol κ	dCTP	11.8 ± 0.6	32 ± 5	3.7 × 10−1
	L-3TC-TP	0.00045 ± 0.00001	4.0 ± 0.5	1.1 × 10−4	3,300
	L-FTC-TP	0.000225 ± 0.000003	4.8 ± 0.2	4.7 × 10−5	7,900
Pol ι	dCTP	0.075 ± 0.002	50 ± 5	1.5 × 10−3
	L-3TC-TP	0.00356 ± 0.00009	96 ± 8	3.7 × 10−5	40
	L-FTC-TP	0.0020 ± 0.0001	230 ± 20	8.7 × 10−6	170
Rev1	dCTPc	22.4 ± 0.9	2.2 ± 0.3	10
	L-3TC-TP	0.0236 ± 0.0006	1.04 ± 0.10	2.3 × 10−2	450
	L-FTC-TP	0.0222 ± 0.0007	2.7 ± 0.3	8.2 × 10−3	1,200
Pol γ	dCTPd	44 ± 2	1.1 ± 0.1	40
	L-3TC-TPd	0.125 ± 0.005	9.2 ± 0.9	1.4 × 10−2	2,900
	L-FTC-TPe	0.0086 ± 0.0015	62.9 ± 8.4	1.4 × 10−4	290,000
HIV-1 RT	dCTPf	2.9 ± 0.2	56 ± 10	5.2 × 10−2
	L-3TC-TPg	0.019 ± 0.001	15 ± 3	1.3 × 10−3	41
	L-FTC-TPe	0.039 ± 0.003	12 ± 3	3.3 × 10−3	16

^aCalculated as [(k_p/K_d)_dCTP]/[(k_p/K_d)_analog].

^bKinetic parameters are from reference 15.

^cKinetic parameters are from reference 6.

^dKinetic parameters are from reference 12.

^eKinetic parameters are from reference 13.

^fKinetic parameters are from reference 44.

^gKinetic parameters are from reference 11.