TABLE 3.

Kinetic parameters for nucleotide incorporation opposite template base dT at 37°C

DNA polymerase	dNTP	kp (s−1)	Kd (μM)	kp/Kd (μM−1 s−1)	Selection factora
Pol β	dATP	32 ± 1	9.2 ± 1.0	3.5
	PMPA-DP	4.7 ± 0.5	50 ± 10	9.4 × 10−2	40
Pol λ	dATPb	1.5 ± 0.1	0.9 ± 0.3	1.7
	PMPA-DP	0.095 ± 0.008	3.7 ± 0.9	2.6 × 10−2	65
Pol η	dATP	35 ± 3	130 ± 26	2.7 × 10−1
	PMPA-DP	0.0134 ± 0.0007	90 ± 10	1.5 × 10−4	1,800
Pol κ	dATP	2.49 ± 0.08	7.0 ± 1.0	3.6 × 10−1
	PMPA-DP	0.010 ± 0.005	3,000 ± 2,000	3.3 × 10−6	110,000
Pol ι	dATP	0.015 ± 0.001	260 ± 40	5.8 × 10−5
	PMPA-DP	Could not measure			High
Rev1	dATP	0.00152 ± 0.00005	2.0 ± 0.3	7.6 × 10−4
	PMPA-DP	Could not measure			High
Pol γ	dATPc	45 ± 1	0.8 ± 0.1	56
	PMPA-DPd	0.21 ± 0.01	40.3 ± 5.7	5.2 × 10−3	10,800
T7 exo	dATPe	156 ± 8	8 ± 2	19.5
	PMPA-DPe	0.096 ± 0.009	268 ± 39	3.6 × 10−4	54,400
HIV-1 RT	dATPe	41.3 ± 0.6	8.1 ± 0.9	5.1
	PMPA-DPe	49 ± 5	58 ± 11	8.4 × 10−1	6

^aCalculated as [(k_p/K_d)_dATP]/[(k_p/K_d)_PMPA-DP].

^bKinetic parameters are from reference 15.

^cKinetic parameters are from reference 22.

^dKinetic parameters are from reference 23.

^eKinetic parameters are from reference 50, and T7 exo was assayed at 20°C.