FIG. 2.

Yeast cells mutant for ARF1 are hypersensitive to gentamicin. (Top) Wild-type (WT) cells (NYY0-1) or cells expressing only the indicated ARF1 mutation (NYY11-2, NYY16-1, or NYY18-1) were first grown overnight in YPD medium at 23°C, then transferred to solid YPD medium containing the indicated concentration of gentamicin, and finally incubated at 23°C overnight. (Bottom) The same strains were transferred to solid YPD medium and were grown overnight. Cells were then transferred to fresh YPD medium and were grown overnight at the indicated temperature, and images were captured. RT, room temperature.