FIG. 5.

Gentamicin disrupts CPY processing in cells mutant for Arf or Arf regulators. Strains were grown at 30°C to early-log phase (5 × 10⁶ cells/ml) before gentamicin (to a final concentration of 500 μg/ml) was added to the medium. After 0, 4, or 8 h of additional incubation at 30°C, aliquots of cells were removed and prepared for Western blot analysis using anti-CPY primary antibodies. Gent, gentamicin.