FIG. 7.

Enrichment of yeast Arf1 and rat Arf1 by gentamicin-Sepharose. (Top) Western blot analysis for yeast Arf1-TAP protein. From left to right, lanes show whole-cell lysate from an ARF1-TAP strain that was added to and incubated with gentamicin-Sepharose; four washes of the column after incubation with whole-cell lysate; and final elution from the column. (Bottom) Western blot analysis of rat Arf1 protein using preimmune serum or anti-rat Arf1 (FabGennix, Frisco, TX). Lanes are the same as explained above, except that the leftmost lane shows a loading control of rat kidney cortex lysates, which were added to and incubated with gentamicin-Sepharose.