FIG. 1.
Subcellular fractionation strategy. Shown is the overall strategy used to disrupt cells and obtain different subcellular fractions for proteomic analysis. The filtrate was purified as the secreted (S) fraction. All cells were lysed by pressing through a French press at 20,000 lb/in2, and a low-speed spin separated microsomal (M) and cytoplasmic (C) fractions from the cell wall/plasma membrane (CW/PM) fraction. A high-speed spin then separated the final two fractions. PM, plasma membrane; ER, endoplasmic reticulum; Mito., mitochondria.