FIG. 3.
Effect of paromomycin on polyphenylalanine synthesis in cell-free systems. (A and B) S30 cell fractions were obtained from the Crithidia fasciculata (•) or Leishmania mexicana (○) trypanosomatid parasites (A) or rat liver (B). (C) Polypeptide synthesis in reaction mixtures containing an S150 supernatant fraction from Leishmania mexicana and purified ribosomes prepared from rat liver (▴) or Leishmania (▪) extracts in the presence of different antibiotic concentrations. Samples were incubated and treated as described in Materials and Methods. All values correspond to the average ± SD of three determinations.