FIG. 4.

Effect of paromomycin on translation misreading. (A) Reaction mixtures containing an S₁₅₀ supernatant fraction from Leishmania mexicana and purified ribosomal particles from trypanosomatid parasites (Crithidia fasciculata or Leishmania mexicana) or rat liver (mammalian cells system) were assayed in the absence and presence of 20 μM paromomycin. RL, Cf, and Lm, rat liver, Crithidia fasciculata, and Leishmania mexicana ribosomes, respectively. (B) The reaction mixtures contained an S₁₅₀ fraction from Leishmania mexicana and purified ribosomes from Leishmania (○) or rat liver (•). Assays were carried out at different paromomycin concentrations. Misreading frequencies shown are the average values ± SD for three experiments.