Skip to main content
. 2010 Oct 18;55(1):86–93. doi: 10.1128/AAC.00506-10

FIG. 5.

FIG. 5.

Surface plasmon resonance analysis (SPR) of the interaction of aminoglycoside antibiotics with L. mexicana rRNA. (A) SPR sensorgram of the interactions between paromomycin (8 to 0.06 mM) and immobilized L. mexicana rRNA (1,450 RU) after correction of nonspecific binding. (B) SPR sensorgram of the interaction of neomycin B (2 to 0.03 mM) and immobilized L. mexicana rRNA (1,560 RU) after correction of nonspecific binding. (C and D) Nonlinear analysis for the determination of the apparent KD of the interaction between immobilized L. mexicana rRNA and aminoglycoside antibiotics, paromomycin, and neomycin B, respectively. (E) SPR sensorgram of the interactions between paromomycin (8 to 0.06 mM) and immobilized human rRNA (1,400 RU). (F) SPR sensorgram of the interactions between ampicillin (8 to 0.06 mM) and immobilized L. mexicana rRNA (1,510 RU). The sensorgrams are representative of at least three different experiments.