TABLE 1.

Oligonucleotide primers used in PCR and DNA sequencing

Resistance gene	Primer	Sequence (5′ → 3′)	Accession no.	Hybridization temp (°C)	PCR product size (bp)	Reference or source
ethA (1,470 bp)	ETHA1	ATC ATC GTC GTC TGA CTA TGG	BX842584	64	1,278	21
	ETHA9	CCT CGA GTA CGT CAA GAG CAC
	ETHA10	CGT TGA CGG CCT CGA CAT TAC		64	342	21
	ETHA8	GGT GGA ACC GGA TAT GCC TG
ethR (651 bp)	ETHR1	CGC TGA CAC CGG AGA TTC C	BX842584	59	915	This study
	ETHR4	CGC TCC TAT ATG ACC GCA CG
ndh (1,392 bp)	Ndh1s	ACT GAG TAC CTG GCA GGC TG	BX842578	57	1,479	This study
	Ndh4as	GCT AAC TGA ACT CGC TCA TCG
mshA (1,443 bp)	mshA5s	GCG TGT CAC TTC GGT TCC TGC	BX842573	57	749	This study
	mshA6as	CAC CGG GAT GGA CCA CGT CG
	mshA11s	GGC CGC CGT GAA GAA CGC GG		62	1,020	This study
	mshA12as	CAG CTC GAC GAT CAG CGC GG
dfrA (480 bp)	dfr2s	CGC TTG CGG GGG ACG AAG C	BX842580	61	480	This study
	dfr4as	GCA TCC GGC GAT CAA AGC TCC
katG (2,223 bp)	katA	CCC GAT AAC ACC TCC TG	BX842578	59	817	5
	katCas	GTT TCG ACG TCG TTC ATG GC
	kat2	CTC GGC GAT GAG CGT TAC AG		59	1,318	5
	kat4as	CCA GCG GTA AGC GCT TGT AG
	katC	CCG AGT ACA TGC TGC TCG AC		59	609	5
	katEas	GGT GAT CGC ACA TCC AGC AC
fabG1-inhA promoter	Pro1	TCA ATA CAC CCG CAG CCA	BX842576	53	493	5
	Pro2	GTC ATC CGC ATG AGG AAT
inhA (810 bp)	INHA1	AGG ACG CAC ATG ACA AGC	BX842576	53	412	5
	INHA2	TCA TGA TCG GCA GCA GCG
	INHA3	CCA CAT CTC GGC GTA TTC		53	601	5
	INHAD	CGA AAT GCA GGT AGT GCT C