FIG. 5.

Initial anti-HIV activity screen. The series (amine, guanide, and phenylguanide) of the most active derivatives (spermidine, spermine, and DNT2300) in the T140 cross-link inhibition assay were screened for anti-HIV activity. TZM-bl cells were plated and preincubated with the inhibitors at a final concentration of 10 μM. Previously titered viral supernatants were added, and the cells were incubated for 3 days. Cells were lysed, and the TAT-driven reporter luciferase activity was measured using a Bright-Glo luciferase assay. Experiments were performed in triplicate with errors shown as means ± the standard error of the mean (SEM). RLU, relative luminescence units.