FIG. 4.
Comparison of glycosylated and aglycosyl CMG2-Fc. Purified CMG2-FcA (2.5 μg/lane, lanes 1 and 3) or CMG2-FcG (2.5 μg/lane, lanes 2 and 4) were electrophoresed through an SDS-polyacrylamide gel. (A) The gel was stained with Coomassie. Additional gels were blotted onto nitrocellulose and probed with anti-human IgG (B) or concanavalin A (C). Lanes 1 and 2, protein reduced with DTT; lanes 3 and 4, nonreduced protein. M, molecular mass standards, indicated in kDa. (D) Toxin neutralization assay. CMG2-FcA, solid black line with solid circles; CMG2-FcG, dotted white line with squares. Each point is the mean of three replicates, and error bars represent the standard errors of the means.