TABLE 1.
Percent viability of Ascaris eggs after exposure to fatty acids for 20 h
| Acid(s) | pKa | Molarity | Percent viability ± SD (pH) of Ascaris eggs at pH rangea |
|||
|---|---|---|---|---|---|---|
| 2.0-4.0 | 4.0-4.5 | 4.5-5.0 | 5.0-6.0 | |||
| Butanoic | 4.76 | 0.1 | 91.6 ± 2.5 (2.0) | 88.4 ± 1.7 a (4.0) | 90.8 ± 1.1 (4.5) | 91.7 ± 1.3 (5.0) |
| 1 | 0.0 ± 0.0 a (2.0) | 0.0 ± 0.1 a (4.0) | 0.1 ± 0.1 a (4.5) | 1.2 ± 0.3 a (5.0) | ||
| 1.5 | 0.0 ± 0.0 a (2.0) | 0.0 ± 0.0 a (4.0) | 0.1 ± 0.1 a (4.5) | 0.0 ± 0.1 a (5.0) | ||
| Pentanoic | 4.78 | 6.3E-06 | 91.8 ± 1.6 (3.5) | 89.9 ± 1.1 | 91.9 ± 0.2 (4.5) | 93.6 ± 1.2 (5.0) |
| 0.01 | 88.4 ± 2.1 a (2.9) | 89.9 ± 1.7 (4.0) | 91.0 ± 2.6 (4.5) | 88.6 ± 2.2 (5.0) | ||
| 0.1 | 86.4 ± 1.0 a (2.7) | 92.7 ± 1.8 (4.0) | 90.1 ± 1.0 (4.5) | 87.1 ± 3.0 a (5.0) | ||
| 0.2 | 0.3 ± 0.3 (2.5) | 0.2 ± 0.1 (4.0) | 90.5 ± 1.4 (4.5) | 90.8 ± 0.5 (5.0) | ||
| Hexanoic | 4.85 | 6.6E-06 | NDb | ND | ND | 89.3 ± 0.5 (5.4) |
| 0.0001 | ND | 91.2 ± 0.7 (4.4) | 88.2 ± 1.4 (4.5) | 89.3 ± 0.5 (5.1) | ||
| 0.001 | 85.9 ± 1.0 (3.4) | 91.1 ± 1.2 (4.0) | 89.2 ± 2.0(4.5) | 90.8 ± 1.8 a (5.0) | ||
| 0.0378 | 0.1 ± 0.2 a (3.1) | 0.3 ± 0.1 a (4.0) | 5.3 ± 0.7 a (4.5) | 91.9 ± 1.9 a (5.0) | ||
| Butanoic and pentanoicc | 4.76/4.78d | 1.36/0.2d | ND | 0.0033 ± 0.0058 b (4.0) | ND | 90.0 ± 3.6 (6.0) |
| 0.136/0.2d | ND | 1.98 ± 0.24 b (4.0) | ND | ND | ||
| 1.36/0.02d | ND | 0.0033 ± 0.0058 b (4.0) | ND | ND | ||
| 0.136/0.02d | ND | 91.38 ± 2.2 (4.0) | ND | ND | ||
a
Letters after viability measurements denote statistically significant differences among the percentages of viable eggs when compared to the water controls which were conducted with each round of testing (data not shown). The overall average percent survival for the water controls was 90.3 ± 2.7%. Data marked “a” were tested with the Proc Mix of SAS (P < 0.01). Data marked “b” were tested with the Student t test (P < 0.05). SD, standard deviation.
b
ND, not done.
c
The concentrations tested were chosen to reflect high concentrations and low concentrations.
d
Values refer to butanoic acid and pentanoic acid, respectively.