TABLE 1.

Virtual PCR-RFLP analysis performed with iPCR and REBsites electronic tools^a

vt gene variant	GenBank accession no.	Predicted size of PCR productsb	Predicted sizes of restriction fragmentsc
			HincII	RsaI
vt1	M19473	898	707, 158, 33	574, 162, 93, 69
vt1c	AJ312232	899	708, 158, 33	354, 221, 162, 93, 69
vt1d	AY170851	897	864, 33	573,162, 93, 69

^aPredicted sizes of PCR products and restriction fragments for vt₁ and vt₂ variants are indicated in bp.

^bCalculated with iPCR software (http://www.ch.embnet.org/software/iPCR_form.html).

^cCalculated using REBsites, a virtual digestion tool (http://tools.neb.com/REBsites).