TABLE 2.
Verotoxin gene (vt) subtyping and verotoxicity results
| Serotypea | No. (origin)b | vt genotypec | eaed | ehxAd | saad | Verotoxicity classification |
|
|---|---|---|---|---|---|---|---|
| Uninduced conditione | Induced conditionf | ||||||
| O2:H− | 1 (h) | vt2vhb | − | − | − | ||
| O2:H5 | 1 (a) | vt2 | − | − | + | ||
| O2:H25 | 1 (f) | vt2g | − | − | − | C | I |
| O5:H− | 4 (c) | vt1 | + | + | − | ||
| O5:H27 | 1 (c) | vt1 | + | + | − | ||
| O8:H16 | 3 (2b, 1f) | vt1 | − | − | + | ||
| O8:H19 | 4 (b) | vt2 | − | + | − | ||
| O15:H21 | 1 (f) | vt2g | − | − | − | A | I |
| O20:H7 | 2 (a) | vt1vt2vhb | − | − | − | ||
| O20:H19 | 2 (a, h) | vt1vt2 | − | + | + | C | II |
| O20:H19 | 1 (f) | vt1vt2 | − | + | + | C | III |
| O20:H19 | 1 (c) | vt1vt2vhb | − | + | + | C | II |
| O20:H19 | 1 (c) | vt1vt2vhb | − | − | − | B | II |
| O20:H19 | 1 (g) | vt2 | − | + | + | C | III |
| O20:H19 | 1 (h) | vt2vhb | − | − | − | B | III |
| O20:H? | 1 (c) | vt1 | + | + | − | ||
| O22:H8 | 2 (h) | vt1vt2 | − | + | + | ||
| O22:H8 | 1 (b) | vt2 | − | − | − | ||
| O25:H19 | 1 (f) | vt2 | − | + | − | ||
| O26:H11 | 3 (c) | vt1 | + | + | − | C | I |
| O26:H11 | 2 (c) | vt2 | + | + | − | C | III |
| O26:H11 | 2 (c) | vt1 | + | + | − | C | III |
| O38:H39 | 1 (c) | vt1 | + | + | − | ||
| O39:H49 | 1 (a) | vt1vt2 | − | + | + | ||
| O39:H49 | 4 (a) | vt2 | − | + | + | ||
| O74:H28 | 1 (a) | vt2vt2vhb | − | + | + | ||
| O79:H19 | 1 (a) | vt2vhb | − | + | + | ||
| O88:H21 | 1 (h) | vt1vt2 | − | + | + | ||
| O91:H21 | 2 (f) | vt2 | − | + | + | C | III |
| O91:H21 | 1 (h) | vt2vt2vhb | − | + | + | B | I |
| O91:H21 | 1 (g) | vt2vhb | − | + | + | B | I |
| O103:H− | 1 (c) | vt1vt2 | + | + | − | C | III |
| O103:H− | 1 (a) | vt2vt2vhb | + | + | − | ||
| O103:H2 | 1 (f) | vt1 | + | + | − | ||
| O103:H2 | 1 (c) | vt1vt2 | + | + | − | ||
| O111:H− | 1 (c) | vt1 | + | + | − | ||
| O112:H2 | 1 (b) | vt2vhbvt2O118 | − | − | − | ||
| O113:H− | 1 (b) | vt2vt2vha | − | − | − | ||
| O113:H21 | 1 (e) | vt2 | − | + | + | B | II |
| O113:H21 | 1 (b) | vt2 | − | + | + | C | III |
| O113:H21 | 1 (g) | vt2vt2vhavt2vhb | − | + | + | C | III |
| O113:H21 | 2 (h, f) | vt2vha | − | − | − | A | II |
| O113:H21 | 1 (f) | vt2vha | − | − | − | ||
| O116:H21 | 2 (b, g) | vt2 | − | + | + | ||
| O117:H7 | 5 (1a, 1g, 2h, 1f) | vt2vha | − | − | − | ||
| O117:H7 | 3 (f) | vt2vhb | − | − | − | ||
| O118:H16 | 1 (c) | vt1 | + | + | − | ||
| O120:H19 | 1 (f) | vt2 | − | + | + | ||
| O141:H7 | 1 (a) | vt1vt2 | − | + | + | ||
| O141:H8 | 1 (g) | vt1vt2 | − | + | + | ||
| O141:H8 | 1 (g) | vt2 | − | + | + | ||
| O145:H− | 1 (f) | vt1 | + | + | − | ||
| O145:H− | 1 (f) | vt1 | + | + | − | A | I |
| O145:H− | 1 (f) | vt1 | + | − | − | A | I |
| O145:H− | 2 (f) | vt1 | + | + | − | B | I |
| O145:H− | 4 (3f, 1a) | vt2 | + | + | − | C | III |
| O145:H− | 1 (f) | vt2 | + | − | − | C | III |
| O145:H− | 2 (f) | vt2 | + | + | − | ||
| O146:H− | 1 (f) | vt2 | + | + | − | ||
| O146:H21 | 1 (f) | vt2 | + | − | − | ||
| O157:H7 | 5 (4f, 1h) | vt2vt2vha | + | + | − | C | III |
| O157:H7 | 1 (f) | vt2vt2vha | + | + | − | B | I |
| O162:H7 | 1 (h) | vt2vha | − | − | − | ||
| O165:H− | 1 (c) | vt2vt2vha | + | + | − | ||
| O168:H8 | 1 (a) | vt2vhb | − | − | − | ||
| O171:H− | 3 (f) | vt2vhb | − | − | − | ||
| O171:H− | 1 (b) | vt2vhbvt2O118 | − | − | − | ||
| O171:H2 | 2 (f) | vt2vha | − | − | − | ||
| O171:H2 | 1 (a) | vt2vhavt2O118 | − | − | − | ||
| O171:H2 | 2 (a) | vt2vhbvt2O118 | − | − | − | ||
| O171:H2 | 7 (1b, 1a, 5f) | vt2vhb | − | − | − | ||
| O171:H? | 1 (b) | vt2vt2vhbvt2O118 | − | − | − | ||
| O174:H21 | 1 (f) | vt1 | − | − | − | B | II |
| O174:H21 | 1 (a) | vt1vt2vt2vhb | − | + | + | A | I |
| O174:H21 | 1 (a) | vt2vhavt2vhb | − | − | − | B | II |
| O174:H21 | 1 (a) | vt2vhb | − | − | − | A | I |
| O174:H21 | 1 (f) | vt2vhb | − | − | − | B | III |
| O174:H21 | 1 (f) | vt2vhb | − | − | − | A | III |
| O174:H21 | 1 (f) | vt2vhb | − | − | − | A | II |
| O174:H21 | 4 (1c, 2b, 1f) | vt2vhb | − | − | − | ||
| O175:H8 | 2 (f) | vt2g | − | − | − | A | I |
| O177:H− | 2 (f) | vt2vha | + | + | − | ||
| O177:H− | 1 (c) | vt2vhb | + | + | − | ||
| O178:H19 | 1 (b) | vt2 | − | + | + | B | III |
| O178:H19 | 2 (1b, 1f) | vt2vha | − | − | − | B | III |
| O178:H19 | 1 (f) | vt2vhb | − | − | − | A | II |
| O185:H7 | 1 (b) | vt2vhb | − | − | − | ||
| ONT:H− | 1 (b) | vt1vt2vt2vhbvt2O118 | − | + | − | ||
| ONT:H− | 15 (6a, 9f) | vt2 | − | − | − | ||
| ONT:H− | 1 (f) | vt2vhb | − | − | − | ||
| ONT:H7 | 2 (b) | vt2vha | − | − | − | ||
| ONT:H7 | 4 (3b, 1h) | vt2vhb | − | − | − | ||
| ONT:H7 | 1 (b) | vt2vhb | − | + | + | ||
| ONT:H8 | 1 (a) | vt1d | − | − | − | ||
| ONT:H8 | 1 (h) | vt1vt2 | − | + | + | ||
| ONT:H19 | 1 (b) | vt1vt2 | − | + | + | ||
| ONT:H19 | 1 (b) | vt2 | − | + | + | ||
| ONT:H19 | 2 (b) | vt2vhb | − | + | + | ||
| ONT:H19 | 1 (b) | vt2vhb | − | + | + | ||
| ONT:H21 | 1 (f) | vt1vt2 | − | + | + | ||
| ONT:H21 | 1 (e) | vt2 | − | + | + | ||
| ONT:H21 | 1 (c) | vt2vhavt2vhb | − | − | − | ||
| ONT:H21 | 10 (3a, 1c, 3b, 3f) | vt2vhb | − | − | − | ||
| ONT:H21 | 1 (f) | vt2vhb | − | + | − | ||
| ONT:HNT | 1 (b) | vt2vhb | − | − | − | ||
| ONT:HNT | 1 (h) | vt2vt2vhb | − | + | + | ||
| O157:H7 | Reference straing | vt1vt2 | + | + | − | C | III |
a
Isolates presenting the same serotype and virulence profile (in regard to vt genotype and the presence/absence of eae and ehxA genes) have been isolated from humans with bloody diarrhea and HUS in Argentina (47, 48) are in boldface.
b
a, cattle at abattoir; b, ground beef; c, calf; e, evisceration tray (at abattoir); f, cattle in feedlot; g, grazing cattle; h, hamburger.
c
Underlined areas indicate the mucus-activatable genotype.
d
Presence of vt1, vt2, ehxA, and eae genes was determined by multiplex PCR analysis (40).
e
Mean titers were classified in three categories: A, ≤ 16; B, 17 to 255; C, ≥256.
f
Mean titers were classified in three categories: I, ≤4,096; II, 4,097 to 131,071; III, ≥131,072.
g
The control strain was E. coli EDL933.