FIG. 5.

Effects of pH and temperature on the activity and stability of Xyl6E7. (A) The pH range of Xyl6E7 was assayed at 55°C between pH 4.0 and 10.5. (B) pH stability was measured after preincubation in buffers ranging from pH 4.5 to 10 at 4°C for 6 days. (C) The temperature range was assayed between 20°C and 70°C at pH 7.5. (D) Thermostability was detected by measuring the residual activity after preincubation at 30°C to 50°C for 5 min, 10 min, and 30 min. All relative activity values were obtained from triplicate experiments. For more details, refer to Materials and Methods.