TABLE 1.

Virus primers and probes

Microorganism	GenBank accession no.	Primer or probe	Primer/probe final concn (nM)	Probe labela	Sequenceb	Product size (bp)	Product regionc	Reference(s)
Murine norovirus	AY228235	MNVKS1	400		5′ AGGTCATGCGAGATCAGCTT 3′	159	ORF1	3
		MNVKS2	400		5′ CCAAGCTCTCACAAGCCTTC 3′
		MNVKS3	200	FAM	5′ CAGTCTGCGACGCCATTGAGAA 3′
Human norovirus GI	M87661	COG1F	1,000		5′ CGYTGGATGCGNTTYCATGA 3′	85	ORF1-ORF2	22
		COG1R	1,000		5′ CTTAGACGCCATCATCATTYAC 3′		junction
		RING 1A	100	FAM	5′ AGATYGCGATCYCCTGTCCA 3′
		RING 1B	100	FAM	5′ AGATCGCGGTCTCCTGTCCA 3′
Human norovirus GII	AF145896	COG2F	1,000		5′ CARGARBCNATGTTYAGRTGGATGAG 3′	88	ORF1-ORF2 junction	22
		COG2R	1,000		5′ TCGACGCCATCTTCATTCACA 3′
		RING2-TP	200	FAM	5′ TGGGAGGGCGATCGCAATCT 3′
Human adenovirus	AC_000008	JTVXF	400		5′ GGACGCCTCGGAGTACCTGAG 3′	96	Hexon	21
(Type A-F)		JTVXR	400		5′ ACIGTGGGGTTTCTGAACTTGTT 3′		region
		JTVXP	150	FAM	5′ CTGGTGCAGTTCGCCCGTGCCA 3′
Human polyomavirus		SM2	500		5′ AGTCTTTAGGGTCTTCTACCTTT 3′	173 (JC), 176	Partial	28
(JC and BK)		P6	500		5′ GGTGCCAACCTATGGAACAG 3′	(BK)	T antigen
		KGJ3	400	FAM	5′ TCATCACTGGCAAACAT 3′
Human enteroviruses	AJ293918	EV1R	700		5′ TGTCACCATAAGCAGCCA 3′	143	5′ UTR	15
		EV1F	700		5′ CCCTGAATGCGGCTAAT 3′
		EV probe	120	FAM	5′ ACGGACACCCAAAGTAGTCGGTTC 3′
Hepatitis G virus	U44402	HepG-F	400		5′ CGGCCAAAAGGTGGTGGATG 3′	185	5′ UTR	24, 36
		HepG-R	400		5′ CGACGAGCCTGACGTCGGG 3′
		HepG probe	200	FAM	5′ AGGTCCCTCTGGCGCTTGTGGCGAG 3′

^aThe FAM (6-carboxyfluorescein) quencher is BHQ-1 (Black Hole Quencher). The FAM quencher is a minor groove binder nonfluorescent quencher (MGBNFQ).

^bMixed bases in degenerate primers and probes are as follows: Y = C, T; R = A, G; B = C, T, G; N = A, C, T, G.

^cORF, open reading frame; UTR, untranslated region.