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. 2010 Nov 5;77(1):343–345. doi: 10.1128/AEM.01872-10

TABLE 1.

Summary of analysis results of STEC strains isolated from produce

Strain Serotypea Source Results of analysis
PCRb Stx2c EhxAd saae subABf sabg
09-00002 NA Spinach stx2, ehxA + + + + NAh
09-00009 O172:H2 Spinach stx2 NA NA
09-00024 O116:H21 Spinach stx2, ehxA + + + + NA
09-00025 O116:H21 Spinachi stx2, ehxA + + + + NA
09-00026 O116:H21 Spinachi stx2 + NA NA
09-00027 O113:H? Spinach stx2, ehxA + + + +
09-00028 O8:H− Spinach stx2, ehxA Weak + NA
09-00029 O11:H15 Spinach stx2? NA NA
09-00031 O−:H19 Spinach stx2, ehxA + + + + NA
09-00039 O168:H8 Spinach stx2, ehxA + NA
09-00043 O−:H21 Spinach stx2 NA NA
09-00047 O113:H21 Spinach stx2, ehxA + + + +
09-00049 O168:H− Lettuce stx2, ehxA + NA
a

O and H serotyping results from the E. coli Reference Center, Penn State University.

b

Various PCRs were used to test for stx1 and stx2, ehxA (enterohemolysin), γ-eae, and other eae alleles, and for O antigen genes for O157, O26, O111, O121, O145, and O103 serotypes.

c

Serological analysis was done to test for Stx2 using VTEC-RPLA (Denka Seiken, Japan).

d

Enterohemolysis was done on blood agar plates with Ca2+ to test for EhxA.

e

PCR was used to test for the Saa gene.

f

PCR was used to test for the subtilase cytotoxin genes.

g

PCR was used to test for the gene encoding the STEC autotransporter protein.

h

NA, not applicable.

i

Organic product.

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