TABLE 1.
Sequences of primers used in RT-PCR amplification, their annealing temperatures, and expected PCR product sizes
| Target gene | Primera | Sequence (5′-3′) | Ta (°C)b | Size (bp) |
|---|---|---|---|---|
| Prochlorococcus sp. MED4 | ||||
| cynA | RTcA-Med4 F | GGAGGTAGCTAAGGCTATTT | 52.5 | 198 |
| RTcA-Med4 R | CCTCCTAGATCCCATCTTAT | |||
| cynS | RTcS-Med4 F | CCTACGGATCCTCTTATCTA | 52 | 163 |
| RTcS-Med4 R | CTAGAACCCTATCTCCCTTT | |||
| ntcA | RTnA-Med4 F | AGAGGAGCAGTAAGGTTATC | 51 | 116 |
| RTnA-Med4 R | TCAGACCTATGTCCTGTTAG | |||
| PMM0615 | RTorf1206 F | CCCTGAACTTTATAGACACC | 52 | 202 |
| RTorf1206 R | GACTTTGTCTTCTCCCATAG | |||
| Synechococcus sp. WH8102 | ||||
| cynA | cA-8102 F | GCCTCTATTCACTCTAGTTCCCC | 53 | 828 |
| cA-8102 R | GCGAATTATGCAACAAATCCTACT | |||
| cynS | cS-8102 F | AGGTTTGGGTTGCATCTTTG | 52.5 | 235 |
| cS-8102 R | TCTCCGAAATGCTCCTGAAT | |||
| 16S rRNA | 16S-8102 F | CATCATGCCCCTTACATCCT | 56 | 103 |
| 16S-8102 R | AACTGAGCCACGGTTTATGG | |||
| Synechococcus sp. WH7803 | ||||
| cynS | RTcS-2 F | GGCCACAGCATCAGCGGAGG | 63 | 283 |
| RTcS-2 R | GGTGATCTTCACGCGATCGCC | |||
| orf0252 | RTorf0252 F | GTGCCCGGTATGTCTTCCTT | 59 | 150 |
| RTorf0252 R | ATTGGCGTCTGTGTTGAGGT |
a
F, forward; R, reverse.
b
Ta, annealing temperature.