TABLE 2.
Comparisons between bovine gastrointestinal and BDD treponemes
| Strain | Isolation material | Nearest relative (% shared identity) | Growth condition (days of growth)a | Presence of: |
Reference | |||
|---|---|---|---|---|---|---|---|---|
| Hemolysis | Serum dependence | tlyC geneb | nifH geneb | |||||
| OC1 | GI tract | T. bryantii (90.0) | NSSR (2-3) | − | − | − | − | This study |
| AC3 | GI tract | T. zioleckii (89.3) | NSSR (2-3) | − | − | − | + | This study |
| CC2 | GI tract | T. bryantii (90.0) | NSSR (2-3) | − | − | − | − | This study |
| CHPA | GI tract | T. bryantii (87.7) | NSSR (2-3) | − | − | − | − | This study |
| RE1 | GI tract | T. bryantii (90.0) | NSSR (2-3) | − | − | − | − | This study |
| RU1 | GI tract | T. zioleckii (90.2) | NSSR (2-3) | − | − | − | + | This study |
| RU2 | GI tract | T. bryantii (90.0) | NSSR (2-3) | − | − | − | − | This study |
| T19 | BDD lesion | T. medium (99.6) | RS (9) | + | + | + | − | 12 |
| T56 | BDD lesion | T. medium (99.6) | RS (9) | + | + | + | − | 12 |
| T320A | BDD lesion | T. phagedenis (100) | FCS (7) | − | + | + | − | 12 |
| T354A | BDD lesion | T. phagedenis (100) | FCS (7) | − | + | + | − | 12 |
| T3552B | BDD lesion | T. denticola (95.7) | FCS (4) | + | + | + | − | 12 |
| G819CN | BDD lesion | T. denticola (95.7) | FCS (4) | + | + | + | − | 12 |
The preferred OTEB serum supplement for optimal growth is described (determined by total cell numbers). The number of days for optimal growth is shown in parentheses. NSSR, no serum supplement required.
Presence as identified by PCR using genus/eubacterium-specific degenerate primers.