TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| DH5α | Escherichia coli DH5αMCR | Invitrogen, Carlsbad, CA |
| 14028 | Salmonella enterica subsp. enterica serovar Typhimurium | ATCC |
| Paratyphi B | S. enterica subsp. enterica serovar Paratyphi B | ATCC |
| Newport | S. enterica subsp. enterica serovar Newport | ATCC |
| Virchow | S. enterica subsp. enterica serovar Virchow | ATCC |
| Gallinarum | S. enterica subsp. enterica serovar Gallinarum 287/91 | 57 |
| Choleraesuis | S. enterica subsp. enterica serovar Choleraesuis | ATCC |
| Arizonae | S. enterica subsp. arizonae | ATCC |
| 14028ΔpocR | In-frame pocR (STM2036) deletion mutant | This study |
| 14028ΔeutR | In-frame eutR (STM2454) deletion mutant | This study |
| 14028ΔrhaSR | In-frame rhaSR (STM4048-STM4049) deletion mutant | This study |
| Plasmids | ||
| pKD4 | Kanr; pir dependent, FRT sites | CGSC, Yale |
| pKD46 | Lambda red helper plasmid; Ampr | CGSC, Yale |
| pCP20 | FLP recombinase plasmid; Cmr Ampr | CGSC, Yale |
| pDEW201 | Promoter probe vector; Ampr; luxCDABE | 60 |
| pDEW-PpduA | pDEW201 with 250 bp upstream of pduA (STM2038) | This study |
| pDEW-PpocR | PDEW201 with 250 bp upstream of pocR (STM2036) | This study |
| pDEW-PpduF | PDEW201 with 250 bp upstream of pduF (STM2037) | This study |
| pDEW-PeutS | PDEW201 with 250 bp upstream of eutS (STM2470) | This study |
| pDEW-PargS | PDEW201 with 244 bp upstream of argS | 32 |
| pDEW-‵STM0047′ | pDEW201 with 350-bp intragenic fragment of STM0047 | 32 |