TABLE 4.
Quantification of promoter activities in the presence of MM with glucose or ethanolamine
| Fragment cloned into pDEW201 | Promoter activitya in 14028 grown in: |
||||
|---|---|---|---|---|---|
| M9 + glucose |
M9 + ethanolamine |
||||
| RLU/OD600 | SD (%) | RLU/OD600 | SD (%) | Fold induction | |
| PpduA | 2.7 × 103 | 20 | 6.5 × 104 | 9 | 24.1 |
| PeutS | 8.5 × 103 | 10.3 | 1.8 × 107 | 3.4 | 2118 |
| PpduF | 7.7 × 102 | 19 | 7.7 × 104 | 13 | 100 |
| PpocR | 5.2 × 102 | 42 | 9.3 × 103 | 12.6 | 17.8 |
| PargS | 7.4 × 105 | 5.2 | 3.4 × 106 | 9 | 4.6 |
| ‵STM0047′ | 5.3 × 103 | 4.4 | 1.5 × 104 | 13 | 2.8 |
a
Gene expression was quantified as relative light units per optical density at 600 nm (RLU/OD600). The highest expression values were taken. Average data from three independent experiments are shown. Fold induction was calculated with respect to the RLU/OD600 values of strain 14028 grown in glucose.