TABLE 5.

Quantification of promoter activities in the presence of MM with CMP or the fat fraction of milk

Fragment cloned into pDEW201	Promoter activitya in 14028 grown in:
	M9 + milk CMP fraction (1%)			M9 + milk fat fraction (1%)			Pasteurized milk with 0.1%/1.5% fat			Pasteurized milk with 3.5% fat
	RLU/OD600	SD (%)	Fold induction	RLU/OD600	SD (%)	Fold induction	RLU/OD600	SD (%)	Fold induction	RLU/OD600	SD (%)	Fold induction
PpduA	BT	BT		1.8 × 102	28	0.2
PeutS	1.65 × 102	13	1.0	8.4 × 104	7.3	11	2.2 × 105/1.6 × 105	5/5	62/46	1.5 × 106	3.6	428
PpduF	BT	BT		1.4 × 102	22	0.2
PpocR	BT	BT		BT	BT
PargSb	5.7 × 103	0.6	36	8.2 × 104	0.9	103
‵STM0047′c	1.6 × 102	20	1	8.0 × 102	7	1

^aFold induction was calculated with respect to the RLU/OD₆₀₀ values of negative-control strain pDEW-‵STM0047′. CMP, casein macropeptide. BT, below the threshold set by the medium control.

^bPositive control.

^cNegative control.